Nuclear Medicine and Biology
Volume 39, Issue 1 , Pages 3-13 , January 2012

Preparation and preclinical evaluation of 177Lu-nimotuzumab targeting epidermal growth factor receptor overexpressing tumors

Received 6 April 2011 ,Revised 30 June 2011 ,Accepted 3 July 2011.

  • Image Result

    Typical instant thin layer chromatography profile of 177Lu-DOTA-h-R3 (top) and 177Lu-DTPA-h-R3 (bottom).

    Typical instant thin layer chromatography profile of 177Lu-DOTA-h-R3 (top) and 177Lu-DTPA-h-R3 (bottom).

  • Image Result

    Competition binding assay. The dilutions were assayed in triplicate, and the average binding was calculated and plotted.

    Competition binding assay. The dilutions were assayed in triplicate, and the average binding was calculated and plotted.

  • Image Result

    Saturation binding assay of 177Lu-(DOTA)n-h-R3 (n=4–5) and 177Lu-(DTPA)n-h-R3 (n=4–5). The dilutions were assayed in triplicate, and the average binding was calculated and plotted.

    Saturation binding assay of 177Lu-(DOTA)n-h-R3 (n=4–5) and 177Lu-(DTPA)n-h-R3 (n=4–5). The dilutions were assayed in triplicate, and the average binding was calculated and plotted.

  • Image Result

    Biodistribution of 177Lu-h-R3 radioimmunoconjugates in tissue/organs collected at different time points from healthy mice. Values are expressed as mean % injected dose/g (%ID/g)±S.D. in groups of thre

    Biodistribution of 177Lu-h-R3 radioimmunoconjugates in tissue/organs collected at different time points from healthy mice. Values are expressed as mean % injected dose/g (%ID/g)±S.D. in groups of three to four animals for each time point.

  • Image Result
    Biodistribution of the 177Lu-(DOTA)n-h-R3 (n=4–5) radioimmunoconjugate in tissue/organs collected at different time points from A431 tumor-bearing mice. Values are expressed as mean %ID/g±S.D. in grou

    Biodistribution of the 177Lu-(DOTA)n-h-R3 (n=4–5) radioimmunoconjugate in tissue/organs collected at different time points from A431 tumor-bearing mice. Values are expressed as mean %ID/g±S.D. in groups of three to four animals for each time point.

☆ This research was supported by EUREKA Grant No. E08018 and Project NPVII 2B06165 from the Czech Ministry of Education, Youth and Sports.

☆☆ Conflicts of interest statement: The authors declare that they have no conflicts of interest.

PII: S0969-8051(11)00154-5

doi: 10.1016/j.nucmedbio.2011.07.001

Nuclear Medicine and Biology
Volume 39, Issue 1 , Pages 3-13 , January 2012

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