In vivo tracking of ¹¹¹In-labeled bone marrow mesenchymal stem cells in acute brain trauma model

Abstract 

Introduction

This study was to evaluate the in vivo distribution of intravenously transplanted bone marrow-derived mesenchymal stem cells (BMSCs) in an acute brain trauma model by ¹¹¹In-tropolone labeling.

Methods

Rat BMSCs were labeled with 37 MBq ¹¹¹In-tropolone. Their labeling efficiency and in vitro retention rate were measured. The viability and proliferation of labeled BMSCs were evaluated for 14 days after labeling. The biodistribution of ¹¹¹In-labeled BMSCs in trauma models was compared with those of sham-operated rats and normal rats on gamma camera images. The migration of ¹¹¹In-BMSCs to the traumatic brain was evaluated using confocal microscope.

Results

The labeling efficiency of ¹¹¹In-BMSCs was 66±5%, and their retention rate was 85.3% at 1 h after labeling. There was no difference in the number of viable cells between ¹¹¹In-BMSCs and controls at 48 h after labeling. However, the proliferation of ¹¹¹In-BMSCs was inhibited after the third day of labeling, and it did not reach confluency. On gamma camera images, most of the ¹¹¹In-BMSCs uptake was observed in the liver and spleen at the second day of injection. The brain uptake of ¹¹¹In-BMSCs was detected prominently in trauma models (1.4%) than in sham-operated (0.5%) or normal rats (0.3%). Radiolabeled BMSCs were observed at the traumatic brain on the confocal microscope as they have a homing capacity, although its proliferation capacity was suppressed.

Conclusion

Although growth inhibition by ¹¹¹In-labeling need to be evaluated further prior to use in humans, ¹¹¹In-labeled BMSCs are useful for the tracking of intravenously transplanted mesenchymal stem cells in brain disease models.

Keywords: ¹¹¹In-tropolone, Mesenchymal stem cells, Cell tracking, Traumatic brain, Radiotoxicity