Nuclear Medicine and Biology
Volume 37, Issue 2 , Pages 197-204 , February 2010

Uptake of 3-[125I]iodo-α-methyl-l-tyrosine into colon cancer DLD-1 cells: characterization and inhibitory effect of natural amino acids and amino acid-like drugs

  • Naoto Shikano

      Affiliations

    • Department of Radiological Sciences, Ibaraki Prefectural University of Health Sciences, Ami-machi, Inashiki-gun, Ibaraki 300-0394, Japan
    • Corresponding Author InformationCorresponding author. Tel.: +81 29 840 2217; fax: +81 29 840 2317.
  • ,
  • Masato Ogura

      Affiliations

    • Department of Radiological Sciences, Ibaraki Prefectural University of Health Sciences, Ami-machi, Inashiki-gun, Ibaraki 300-0394, Japan
  • ,
  • Hiroyuki Okudaira

      Affiliations

    • School of Health Sciences, Faculty of Medicine, Kanazawa University, Kanazawa 920-0942, Japan
  • ,
  • Syuichi Nakajima

      Affiliations

    • Department of Radiological Sciences, Ibaraki Prefectural University of Health Sciences, Ami-machi, Inashiki-gun, Ibaraki 300-0394, Japan
  • ,
  • Takashi Kotani

      Affiliations

    • Department of Radiological Sciences, Ibaraki Prefectural University of Health Sciences, Ami-machi, Inashiki-gun, Ibaraki 300-0394, Japan
  • ,
  • Masato Kobayashi

      Affiliations

    • School of Health Sciences, Faculty of Medicine, Kanazawa University, Kanazawa 920-0942, Japan
  • ,
  • Shinya Nakazawa

      Affiliations

    • Department of Radiological Sciences, Ibaraki Prefectural University of Health Sciences, Ami-machi, Inashiki-gun, Ibaraki 300-0394, Japan
  • ,
  • Takeshi Baba

      Affiliations

    • Center for Medical Sciences, Ibaraki Prefectural University of Health Sciences, Ami-machi, Inashiki-gun, Ibaraki 300-0394, Japan
  • ,
  • Naoto Yamaguchi

      Affiliations

    • Center for Medical Sciences, Ibaraki Prefectural University of Health Sciences, Ami-machi, Inashiki-gun, Ibaraki 300-0394, Japan
  • ,
  • Nobuo Kubota

      Affiliations

    • Department of Radiological Sciences, Ibaraki Prefectural University of Health Sciences, Ami-machi, Inashiki-gun, Ibaraki 300-0394, Japan
  • ,
  • Yukio Iwamura

      Affiliations

    • Center for Humanities and Sciences, Ibaraki Prefectural University of Health Sciences, Ami-machi, Inashiki-gun, Ibaraki 300-0394, Japan
  • ,
  • Keiichi Kawai

      Affiliations

    • School of Health Sciences, Faculty of Medicine, Kanazawa University, Kanazawa 920-0942, Japan

Received 20 June 2007 ,Revised 7 October 2009 ,Accepted 31 October 2009.

References 

  1. Kawai K, Fujibayashi Y, Saji H, Yonekura Y, Konishi J, Kubodera A, et al. A strategy for study of cerebral amino acid transport using iodine-123-labeled amino acid radiopharmaceutical: 3-iodo-alpha-methyl-l-tyrosine. J Nucl Med. 1991;32:819–824
  2. Kawai K, Fujibayashi Y, Yonekura Y, Konishi J, Saji H, Kubodera A, et al. An artificial amino acid radiopharmaceutical for single photon emission computed tomographic study of pancreatic amino acid transports 123I-3-iod-alpha-methyl-l-tyrosine. Ann Nucl Med. 1992;6:169–175
  3. Kawai K, Fujibayashi Y, Yonekura Y, Tanaka K, Saji H, Konishi J, et al. Canine SPECT studies for cerebral amino acid transport by means of 123I-3-iodo-α-methyl-l-tyrosine and preliminary kinetic analysis. Ann Nucl Med. 1995;9:47–50
  4. Biersack HJ, Coenen HH, Stoecklin G, Reichmann K, Bockische A, Oehr P, et al. Imaging of brain tumors with l-3-[I-123]iodo-α-methyl tyrosine and SPECT. J Nucl Med. 1989;30:110–112
  5. Kuwert T, Woesler B, Morgenroth C, Lerch H, Schafers M, Palkovic S, et al. Diagnosis of recurrent glioma with SPECT and iodine-123-α-methyl tyrosine. J Nucl Med. 1998;39:23–27
  6. Jager PL, Franssen EJF, Kool W, Szabo BG, Hoeckstra HJ, Groen HJM, et al. Feasibility of tumor imaging using l-3-[iodine-123]-iodo-alpha-methyl-tyrosine in extracranial tumors. J Nucl Med. 1998;39:1736–1743
  7. Langen KJ, Pauleit D, Coenen HH. 3-[123I]Iodo-α-methyl-l-tyrosine: uptake mechanisms and clinical applications. Nucl Med Biol. 2002;29:625–631
  8. Langen KJ, Muhlensiepen H, Holschibach M, Hautzel H, Jansen P, Coenen HH. Transport mechanisms of 3-[123I]iodo-α-methyl-l-tyrosine in a human glioma cell line: comparison with [3H-methyl]-l-methionine. J Nucl Med. 2000;4:1250–1255
  9. Reimann B, Stoegbauer F, Kopka K, Halffer H, Lasic M, Schirmachr A, et al. Kinetics of 3-[123I]iodo-l-α-methyl tyrosine transport in rat C6 glioma cells. Eur J Nucl Med. 1999;26:1274–1278
  10. Riemann B, Kopka K, Stogbauer F, Halfter H, Ketterler S, Vu Phan TQ, et al. Kinetic parameters of 3-[123I]iodo-l-α-methyl tyrosine ([123I]IMT) transport in human GOS3 glioma cells. Nucl Med Biol. 2001;28:293–297
  11. Franzius C, Kopka K, Valen F, Eckervogt V, Riemann B, Sciuk J, et al. Characterization of 3-[123I]iodo-l-α-methyl tyrosine transport ([123I]IMT) transport into human Ewing's sarcoma cells in vitro. Nucl Med Biol. 2001;28:123–128
  12. Matte G, Sangster SM, Acker M, Hudgins M, Too CK. Characterization of 123I-iodo-alpha-methyltyrosine transport in rat lymphoma cells. Nucl Med Biol. 2005;32:67–73
  13. Shikano N, Kawai K, Nakajima S, Kubodera A, Kubota N, Ishikawa N, et al. Transcellular transport of radioiodinated 3-iodo-alpha-methyl-l-tyrosine across monolayers of kidney epithelial cell line LLC-PK1. Ann Nucl Med. 2004;18:227–234
  14. Prante O, Deichen JT, Hocke C, Kuwert T. Characterization of uptake of 3-[131I]iodo-alpha-methyl-l-tyrosine in human monocyte-macrophages. Nucl Med Biol. 2004;31:365–372
  15. Jager PL, de Vries EG, Piers DA, Timmer-Bosscha H. Uptake mechanisms of l-3-[125I]iodo-alpha-methyl-tyrosine in a human small-cell lung cancer cell line: comparison with l-1. Nucl Med Commun. 2001;22:87–96
  16. Samnick S, Schaefer A, Siebert S, Richter S, Vollmar B, Kirsch CM. Preparation and investigation of tumor affinity, uptake kinetic and transport mechanism of iodine-123-labelled amino acid derivatives in human pancreatic carcinoma and glioblastoma cells. Nucl Med Biol. 2001;28:13–23
  17. Lahoutte T, Caveliers V, Dierickx L, Vekeman M, Everaert H, Mertens J, et al. In vitro characterization of the influx of 3-[125I]iodo-l-alpha-methyltyrosine and 2-[125I]iodo-l-tyrosine into U266 human myeloma cells: evidence for system T transport. Nucl Med Biol. 2001;28:129–134
  18. Yanagida O, Kanai Y, Chairoungdua A, Kim DK, Segawa H, Nii T, et al. Human l-type amino acid transporter 1 (LAT1): characterization of function and expression in tumor cell lines. Biochem Biophys Acta. 2001;1514:291–302
  19. Verrey F, Closs EI, Wagner CA, Palacin M, Endou H, Kanai Y. CATs and HATs: the SLC7 family of amino acid transporters. Pflugers Arch. 2004;447:532–542
  20. Christensen HN. Role of amino acid transport and counter-transport in nutrition and metabolism. Physiol Rev. 1990;70:43–77
  21. Furner RL, Brown RK. l-Phenylalanine mustard (l-PAM): the first 25 years. Cancer Treat Rep. 1980;64:559–574
  22. Usui T, Nagumo Y, Watanabe A, Kubota T, Komatsu K, Kobayashi J, et al. Brasilicardin A, a natural immunosuppressant, targets amino acid transport system L. Chem Biol. 2006;13:1153–1160
  23. Goldstein DS. l-Dihydroxyphenylserine (l-DOPS): a norepinephrine prodrug. Cardiovasc Drug Rev. 2006;24:189–203
  24. Yamamoto H, Kuki M, Ozaki M. Effects of beta-(p-chlorophenyl)-GABA (baclofen) on response to noxious stimuli. Nippon Yakurigaku Zasshi. 1977;73:703–715
  25. Taylor CP, Gee NS, Su TZ, Kocsis JD, Welty DF, Brown JP, et al. A summary of mechanistic hypotheses of gabapentin pharmacology. Epilepsy Res. 1998;29:233–249
  26. Gesellchen V, Kuttenkeuler D, Steckel M, Pelte N, Boutros M. An RNA interference screen identifies inhibitor of apoptosis protein 2 as a regulator of innate immune signalling in Drosophila. EMBO Rep. 2005;6:979–984
  27. Haase C, Bergmann R, Fuechtner F, Hoepping A, Pietzsch J. l-Type amino acid transporters LAT1 and LAT4 in cancer: uptake of 3-O-methyl-6-18F-fluoro-l-dopa in human adenocarcinoma and squamous cell carcinoma in vitro and in vivo. J Nucl Med. 2007;48:2063–2071
  28. Dexter DL, Barbosa JA, Calabresi P. N,N-Dimethylformamide-induced alteration of cell culture characteristics and loss of tumorigenicity in cultured human colon carcinoma cells. Cancer Res. 1979;39:1020–1025
  29. Shikano N, Kanai Y, Kawai K, Ishikawa N, Endou H. Characterization of 3-[125I]iodo-α-methyl-l-tyrosine transport via human l-type amino acid transporter 1. Nucl Med Biol. 2003;30:31–37
  30. Kanai Y, Hediger MA. The glutamate/neutral amino acid transporter family SLC1: molecular, physiological and pharmacological aspects. Pflugers Arch. 2004;447:469–479
  31. Shikano N, Kanai Y, Kawai K, Inatomi J, Kim DK, Ishikawa N, et al. Isoform selectivity of 3-125I-iodo-α-methyl-l-tyrosine membrane transport in human l-type amino acid transporters. J Nucl Med. 2003;44:244–246
  32. Rotmann A, Strand D, Martiné U, Closs EI. Protein kinase C activation promotes the internalization of the human cationic amino acid transporter hCAT-1. A new regulatory mechanism for hCAT-1 activity. J Biol Chem. 2004;279:54185–54192
  33. Sato H, Tamba M, Kuriyama-Matsumura K, Okuno S, Bannai S. Molecular cloning and expression of human xCT, the light chain of amino acid transport system xc. Antioxid Redox Signal. 2000;2:665–671
  34. Shim BS, Kang BH, Hong YK, Kim HK, Lee IH, Lee SY, et al. The kringle domain of tissue-type plasminogen activator inhibits in vivo tumor growth. Biochem Biophys Res Commun. 2005;327:1155–1162

 This work was supported by Grants-in-Aid for Scientific Research (#10770451, #14770498, #13557075, #15659283, #16659322 and #17390336) from the Ministry of Education, Science, Sports and Culture of Japan and the Japan Society for the Promotion of Science. Financial support was also provided by the Ibaraki Prefectural University Research Project (9808, 0118, and 0220); Ibaraki Prefectural University Grants-in-Aid of the Encouragement for Young Scientists 2001, 2002, 2004, 2005, 2006, 2008 and 2009; and Japan Atherosclerosis Research Foundation Grant-2008.

PII: S0969-8051(09)00259-5

doi: 10.1016/j.nucmedbio.2009.10.011

Nuclear Medicine and Biology
Volume 37, Issue 2 , Pages 197-204 , February 2010