Nuclear Medicine and Biology
Volume 37, Issue 1 , Pages 67-72, January 2010

Ultrafast LC method for purification and metabolite analysis of PET probes

  • Ryuji Nakao

      Affiliations

    • Molecular Imaging Center, National Institute of Radiological Sciences, Chiba 263-8555, Japan
    • Corresponding Author InformationCorresponding author. Tel.: +81 43 206 4680; fax: +81 43 206 3261.
    • ,
  • Takehito Ito

      Affiliations

    • Molecular Imaging Center, National Institute of Radiological Sciences, Chiba 263-8555, Japan
    • Sumitomo Accelerator Service, Tokyo 141-8686, Japan
    • ,
  • Kazutaka Hayashi

      Affiliations

    • Molecular Imaging Center, National Institute of Radiological Sciences, Chiba 263-8555, Japan
    • ,
  • Toshimitsu Fukumura

      Affiliations

    • Molecular Imaging Center, National Institute of Radiological Sciences, Chiba 263-8555, Japan
    • ,
  • Kazutoshi Suzuki

      Affiliations

    • Molecular Imaging Center, National Institute of Radiological Sciences, Chiba 263-8555, Japan

Received 8 July 2009; received in revised form 5 August 2009; accepted 8 August 2009. published online 05 October 2009.

Abstract 

An ultrafast and efficient high-performance liquid chromatographic (LC) method was developed to purify positron emission tomography (PET) radiopharmaceuticals as well as for metabolite analysis of the plasma sample. Chromatographic separation was achieved on a short (60 mm length) semipreparative (10 mm I.D.) column packed with 2.5-μm particles using a mixture of acetonitrile and sodium phosphate buffer as the mobile phase at a flow rate of 8–10 ml/min. Under the optimum conditions, excellent separation of the target PET probe was obtained from chemical/radiochemical impurities or radioactive metabolites with a very short run time of 2 min. This characteristic enabled significant shortening of the purification and evaporation processes in the production of short-lived radiopharmaceuticals and highly sensitive radiometric analysis with good temporal resolution during the metabolism study.

Keywords: PET, Ultrafast LC, Purification, Metabolite analysis, Short-lived radiopharmaceuticals

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PII: S0969-8051(09)00212-1

doi:10.1016/j.nucmedbio.2009.08.002

Nuclear Medicine and Biology
Volume 37, Issue 1 , Pages 67-72, January 2010

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