Reproducibility of the kinetic analysis of 3′-deoxy-3′-[18F]fluorothymidine positron emission tomography in mouse tumor models

Choi, Seung Jin; Kim, Seog Young; Kim, Su Jin; Lee, Jae Sung; Lee, Sang Ju; Park, Soo Ah; Lee, Seung Jin; Yun, Sung-Cheol; Im, Ki Chun; Oh, Seung Jun; Kim, Sang-We; Kim, Jae Seung; Ryu, Jin Sook; Moon, Dae Hyuk

doi:10.1016/j.nucmedbio.2009.06.001

« Previous Next »Nuclear Medicine and Biology
Volume 36, Issue 7 , Pages 711-719, October 2009

Reproducibility of the kinetic analysis of 3′-deoxy-3′-[¹⁸F]fluorothymidine positron emission tomography in mouse tumor models

Seung Jin Choi
- Department of Nuclear Medicine, Asan Medical Center, University of Ulsan College of Medicine, Seoul, South Korea
Seog Young Kim
- Department of Nuclear Medicine, Asan Medical Center, University of Ulsan College of Medicine, Seoul, South Korea
Su Jin Kim
- Department of Nuclear Medicine, Seoul National University College of Medicine, Seoul, South Korea
Jae Sung Lee
- Department of Nuclear Medicine, Seoul National University College of Medicine, Seoul, South Korea
Sang Ju Lee
- Department of Chemistry, Sogang University, Seoul, South Korea
Soo Ah Park
- Molecular Imaging Center, Asan Institute for Life Sciences, Asan Medical Center, Seoul, South Korea
Seung Jin Lee
- Molecular Imaging Center, Asan Institute for Life Sciences, Asan Medical Center, Seoul, South Korea
- Institute for Innovative Cancer Research, Asan Medical Center, Seoul, South Korea
Sung-Cheol Yun
- Department of Preventive Medicine, Center for Medical Research and Information, University of Ulsan College of Medicine, Seoul, South Korea
Ki Chun Im
- Department of Nuclear Medicine, Asan Medical Center, University of Ulsan College of Medicine, Seoul, South Korea
Seung Jun Oh
- Department of Nuclear Medicine, Asan Medical Center, University of Ulsan College of Medicine, Seoul, South Korea
- Molecular Imaging Center, Asan Institute for Life Sciences, Asan Medical Center, Seoul, South Korea
Sang-We Kim
- Division of Oncology, Department of Internal Medicine, Asan Medical Center, University of Ulsan College of Medicine, Seoul, South Korea
Jae Seung Kim
- Department of Nuclear Medicine, Asan Medical Center, University of Ulsan College of Medicine, Seoul, South Korea
- Molecular Imaging Center, Asan Institute for Life Sciences, Asan Medical Center, Seoul, South Korea
Jin Sook Ryu
- Department of Nuclear Medicine, Asan Medical Center, University of Ulsan College of Medicine, Seoul, South Korea
- Molecular Imaging Center, Asan Institute for Life Sciences, Asan Medical Center, Seoul, South Korea
Dae Hyuk Moon
- Department of Nuclear Medicine, Asan Medical Center, University of Ulsan College of Medicine, Seoul, South Korea
- Molecular Imaging Center, Asan Institute for Life Sciences, Asan Medical Center, Seoul, South Korea
- Institute for Innovative Cancer Research, Asan Medical Center, Seoul, South Korea
- Corresponding author. Department of Nuclear Medicine, Asan Medical Center, University of Ulsan College of Medicine, Seoul 138-736, South Korea. Tel.: +82 2 3010 4592; fax: +82 2 3010 4588.

Received 15 December 2008; received in revised form 27 March 2009; accepted 14 June 2009. published online 30 July 2009.

Abstract

Objectives

We assessed the reproducibility of the kinetic analysis of 3′-deoxy-3′-[¹⁸F]fluorothymidine (FLT) positron emission tomography (PET) in A431 human epidermoid carcinoma and murine Lewis lung carcinoma (LLC) tumor models.

Methods

We injected 7.4 MBq of FLT (n=10 for each group) and acquired 2-h dynamic PET images. A second scan was performed 1 day later. We calculated standardized uptake value (SUV), kinetic rate constants, volume of distribution of phosphorylated FLT (V_dm), net influx constant (K_FLT-CA) and influx constant by Patlak graphical analysis (K_FLT-PA). The percent difference between measurements of a parameter was calculated to compare the reproducibilities of different parameters.

Results

FLT phosphorylation was higher in mice with A431 tumors than in mice with LLC tumors (P<.005). Differences in the standard deviations of the percent differences of parameters were statistically significant (P<.001) in each model. In mice with A431 tumors, SUV, V_dm, K_FLT-CA and K_FLT-PA had standard deviations of the percent difference of ≤20%. The most reproducible parameter was K_FLT-PA, although the standard deviation (15.6%) was not statistically different from those of V_dm (15.8%), K_FLT-CA (17.5%) and SUV (18.9%). In mice with LLC tumors, K₁, K₁/k₂ and k₃ had standard deviations of the percent difference of ≤20%. No macroparameters reflecting a total FLT flux had standard deviations of ≤20%.