Nuclear Medicine and Biology
Volume 36, Issue 6 , Pages 587-590, August 2009

Chelating ion-exchange methods for the preparation of no-carrier-added 64Cu☆

  • Shigeki Watanabe

      Affiliations

    • Plant Positron Imaging Group, Quantum Beam Science Directorate, Japan Atomic Energy Agency, Takasaki, Gunma 370-1292, Japan
    • Corresponding Author InformationCorresponding author. Tel.: +81 27 346 9461; fax: +81 27 346 9688.
    • ,
  • Satoshi Watanabe

      Affiliations

    • Plant Positron Imaging Group, Quantum Beam Science Directorate, Japan Atomic Energy Agency, Takasaki, Gunma 370-1292, Japan
    • ,
  • Jixin Liang

      Affiliations

    • Plant Positron Imaging Group, Quantum Beam Science Directorate, Japan Atomic Energy Agency, Takasaki, Gunma 370-1292, Japan
    • Present address: Department of Isotopes, Chinese Institute of Atomic Energy 102413, Beijing, China.
    • ,
  • Hirofumi Hanaoka

      Affiliations

    • Department of Bioimaging Information Analysis, Gunma University Graduate School of Medicine, Maebashi, Gunma 371-8511, Japan
    • ,
  • Keigo Endo

      Affiliations

    • Department of Diagnostic Radiology and Nuclear Medicine, Gunma University Graduate School of Medicine, Maebashi, Gunma 371-8511, Japan
    • ,
  • Noriko S. Ishioka

      Affiliations

    • Plant Positron Imaging Group, Quantum Beam Science Directorate, Japan Atomic Energy Agency, Takasaki, Gunma 370-1292, Japan

Received 8 January 2009; received in revised form 16 March 2009; accepted 8 April 2009.

Abstract 

Introduction

We have developed a method for producing no-carrier-added 64Cu by using chelating resin bearing iminodiacetic acid groups.

Methods

We optimized the conditions for the selective separation of radioactive Cu from Ni and Co using the chelating resin and produced no-carrier-added 64Cu under the optimized conditions. We analyzed the amounts of the metal ions present in 64Cu by inductively coupled-plasma mass spectroscopy (ICP-MS) and optical emission spectroscopy (ICP-OES), and performed radiolabeling of monoclonal antibodies in order to investigate the quality of the 64Cu produced in this study.

Results

Radioactive Cu was separated from Ni and Co with 0.1 and 2 M HCl solutions. The yield of 64Cu isolated from the 64NiO target was almost 87%. The radiochemical purity of 64Cu obtained from different amounts of 64NiO targets was >99% in all cases. We found that the 64Cu solution presented extremely low amounts of the metal ions and showed high specific activity (average: 595 GBq/μmol). Moreover, the antibodies were labeled with 64Cu with a high average efficiency (average: 88%).

Conclusions

We could efficiently separate 64Cu by using short ion-exchange columns. The chelating ion-exchange method provides a high quality of 64Cu that is sufficient for the synthesis of 64Cu-labeled antibodies and medical applications.

Keywords: No-carrier-added 64Cu, 64NiO, Radiochemical separation, Chelating resin, Specific activity, 64Cu-radiopharmaceutical

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☆ This study was supported in part by a Grant-in-Aid for the 21st Century COE Program from the Ministry of Education, Sports, and Culture of Japan.

PII: S0969-8051(09)00116-4

doi:10.1016/j.nucmedbio.2009.04.005

Nuclear Medicine and Biology
Volume 36, Issue 6 , Pages 587-590, August 2009

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