Nuclear Medicine and Biology
Volume 36, Issue 6 , Pages 605-611, August 2009

Dual-isotope single-photon emission computed tomography for dopamine and serotonin transporters in normal and parkinsonian monkey brains☆

  • I-Hsun Li

      Affiliations

    • Graduate Institute of Medical Sciences, National Defense Medical Center, Taipei 114, Taiwan
    • ,
  • Wen-Sheng Huang

      Affiliations

    • Department of Nuclear Medicine, Tri-Service General Hospital, Taipei, 114, Taiwan
    • ,
  • Chin-Bin Yeh

      Affiliations

    • Department of Psychiatry, Tri-Service General Hospital, Taipei, 114, Taiwan
    • ,
  • Mei-Hsiu Liao

      Affiliations

    • Division of Isotope Application, Institute of Nuclear Energy Research, Taoyaun, 325 Taiwan
    • ,
  • Chia-Chieh Chen

      Affiliations

    • Division of Isotope Application, Institute of Nuclear Energy Research, Taoyaun, 325 Taiwan
    • ,
  • Lie-Hang Shen

      Affiliations

    • Division of Isotope Application, Institute of Nuclear Energy Research, Taoyaun, 325 Taiwan
    • ,
  • Jiang-Chuan Liu

      Affiliations

    • Department of Biology and Anatomy, National Defense Medical Center, Taipei 114, Taiwan
    • ,
  • Kuo-Hsing Ma

      Affiliations

    • Department of Biology and Anatomy, National Defense Medical Center, Taipei 114, Taiwan
    • Corresponding Author InformationCorresponding author. Department of Biology and Anatomy, National Defense Medical Center, Taipei 114, Taiwan. Tel.: +886 2 87923300x18749; fax: +886 2 87923159.

Received 8 September 2008; received in revised form 20 January 2009; accepted 16 March 2009.

Abstract 

Introduction

Parkinson's disease (PD) affects both dopaminergic and serotonergic systems. In this study, we simultaneously evaluated dopamine and serotonin transporters in primates using dual-isotope single-photon emission computed tomography (SPECT) imaging and compared the results with traditional single-isotope imaging.

Methods

Four healthy and one 6-OHDA-induced PD monkeys were used for this study. SPECT was performed over 4 h after individual or simultaneous injection of [99mTc]TRODAT-1 (a dopamine transporter imaging agent) and [123I]ADAM (a serotonin transporter imaging agent).

Results

The results showed that the image quality and uptake ratios in different brain regions were comparable between single- and dual-isotope studies. The striatal [99mTc]TRODAT-1 uptake in the PD monkey was markedly lower than that in normal monkeys. The uptake of [123I]ADAM in the midbrain of the PD monkey was comparable to that in the normal monkeys, but there were decreased uptakes in the thalamus and striatum of the PD monkey.

Conclusions

Our results suggest that dual-isotope SPECT using [99mTc]TRODAT-1 and [123I]ADAM can simultaneously evaluate changes in dopaminergic and serotonergic systems in a PD model.

Keywords: Parkinson's disease, Dopamine transporter, Serotonin transporter, Dual-isotope SPECT, [99mTc]TRODAT-1, [123I]ADAM

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☆ This work was supported by the Institute of Nuclear Energy Research, National Science Council, and Ministry of National Defense under grants 970945L, NSC 96-2623-7-016-001, and DOD 97-24-02, respectively.

PII: S0969-8051(09)00080-8

doi:10.1016/j.nucmedbio.2009.03.003

Nuclear Medicine and Biology
Volume 36, Issue 6 , Pages 605-611, August 2009

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