Nuclear Medicine and Biology
Volume 36, Issue 2 , Pages 183-189 , February 2009

Measurement of glucose metabolism in rat spinal cord slices with dynamic positron autoradiography

  • Xiaoping Fan

      Affiliations

    • Department of Cardiovascular Surgery, Guangdong Cardiovascular Institute, Guangdong Provincial People's Hospital, Guangzhou 510100, China
  • ,
  • Tatsuya Asai

      Affiliations

    • Biomedical Imaging Research Center, University of Fukui, Eiheiji-cho, Fukui 910-1193, Japan
  • ,
  • Koichi Morioka

      Affiliations

    • Department of Cardiovascular Surgery II, University of Fukui, Eiheiji-cho, Fukui 910-1193, Japan
  • ,
  • Kenzo Uchida

      Affiliations

    • Department of Orthopaedics and Rehabilitation Medicine, University of Fukui, Eiheiji-cho, Fukui 910-1193, Japan
  • ,
  • Hisatoshi Baba

      Affiliations

    • Department of Orthopaedics and Rehabilitation Medicine, University of Fukui, Eiheiji-cho, Fukui 910-1193, Japan
  • ,
  • Kuniyoshi Tanaka

      Affiliations

    • Department of Cardiovascular Surgery II, University of Fukui, Eiheiji-cho, Fukui 910-1193, Japan
  • ,
  • Jian Zhuang

      Affiliations

    • Department of Cardiovascular Surgery, Guangdong Cardiovascular Institute, Guangdong Provincial People's Hospital, Guangzhou 510100, China
  • ,
  • Hidehiko Okazawa

      Affiliations

    • Biomedical Imaging Research Center, University of Fukui, Eiheiji-cho, Fukui 910-1193, Japan
  • ,
  • Yasuhisa Fujibayashi

      Affiliations

    • Biomedical Imaging Research Center, University of Fukui, Eiheiji-cho, Fukui 910-1193, Japan
    • Corresponding Author InformationCorresponding author. Tel.: +81 776 61 8491; fax: +81 776 61 8171.

Received 1 October 2008 ,Revised 10 November 2008 ,Accepted 19 November 2008.

References 

  1. Baba H, Uchida K, Sadato N, Yonekura Y, Kamoto Y, Maezawa Y, et al. Potential usefulness of 18-2-fluoro-deoxy-d-glucose-positron emission tomography in cervical compressive myelopathy. Spine. 1999;24:1449–1454
  2. Uchida K, Kobayashi S, Yayama T, Kokubo Y, Nakajima H, Sadato N. Metabolic neuroimaging of the cervical spinal cord in patients with compressive myelopathy: a high-resolution positron emission tomography study. J Neurosurg Spine. 2004;1:72–79
  3. Radu CG, Shu CJ, Shelly SM, Phelps ME, Witte ON. Positron emission tomography with computed tomography imaging of neuroinflammation in experimental autoimmune encephalomyelitis. Proc Natl Acad Sci U S A. 2007;104:1937
  4. Popovich T, Carpenter JS, Rai AT, Carson LV, Wimmiams HJ, Marano GD. Spinal cord compression by tophaceous gout with fluorodeoxyglucose-positron emission tomographic/MR fusion imaging. Am J Neuroradiol. 2006;27:1201
  5. Scremin OU, O'Neal M, Scremin AM. Glucose utilization and reflex activity of the transected rat spinal cord. Brain Res. 1990;531(1-2):203–210
  6. Matsumura K, Bergström M, Onoe H, Takechi H, Westerberg G, Antoni G. In vitro positron emission tomography (PET): use of positron emission tracers in functional imaging in living brain slices. Neurosci Res. 1995;22:219–229
  7. Murata T, Matsumura K, Onoe H, Bergström M, Takechi H, Sihver S. Receptor imaging technique with 11C-labeled receptor ligands in living brain slices: its application to time-resolved imaging and saturation analysis of benzodiazepine receptor using [11C]Ro15-1788. Neurosci Res. 1996;25:145–154
  8. Yamamoto C, Macllwain H. Electrical activities in thin sections from the mammalian brain maintained in chemically defined media in vitro. J Neurochem. 1966;13:1333–1343
  9. Murata T, Omata N, Fujibayashi Y, Waki A, Isaki K, Oka H. Dynamic changes in glucose metabolism of living rat brain slices induced by hypoxia and neurotoxic chemical-loading revealed by positron autoradiography. J Neural Transm. 1999;106:1075–1087
  10. Yoshida S, Murata T, Omata N, Waki A, Fujibayashi Y, Isaki K. The effect of neuronal perturbation on the uptake of [18F]2-fluoro-2-deoxy-d-glucose in brain slices of the rat. Neurosci Res. 1998;30:271–278
  11. Aitken PG, Breese GR, Dudek FF, Edwards F, Espanol MT, Larkman PM, et al. Preparative methods for brain slices: a discussion. J Neurosci Methods. 1995;59:139–149
  12. Richerson GB, Messer C. Effect of composition of experimental solutions on neuronal survival during rat brain slicing. Exp Neurol. 1995;131:133–143
  13. Hori N, Tan Y, Strominger NL, Carpenter DO. Intracellular activity of rat spinal cord motoneurons in slices. J Neurosci Methods. 2001;112:185–191
  14. Carp JS, Tennissen AM, Mongeluzi DL, Dudek CJ, Chen XY, Wolpaw JR. An in vitro protocol for recording from spinal motoneurons of adult rats. J Neurophysiol. 2008;100:474–481
  15. Harkness RA. Is post-hypoxic-ischemic cell damage associated with excessive ATP consumption rather than a failure of ATP production?. Acta Paediatr. 1997;86:1–5
  16. Omata N, Murata T, Maruoka N, Fujibayashi Y, Yonekura Y, Wada Y. Different mechanisms of hypoxic injury on white matter and gray matter as revealed by dynamic changes in glucose metabolism in rats. Neurosci Lett. 2003;353:148–152
  17. Schurr A, Dong WQ, Reid KH, West CA, Rigor BM. Lactic acidosis and recovery of neuronal function following cerebral hypoxia in vitro. Brain Res. 1988;438:311–314
  18. Schurr A, West CA, Rigor BM. Electrophysiology of energy metabolism and neuronal function in the hippocampal slice preparation. J Neurosci Methods. 1989;28:7–13
  19. Newman GC, Hospod FE, Patlak CS. Brain slice glucose utilization. J Neurochem. 1988;51:1783–1796
  20. Edwards F, Konnerth A, Sakmann B. Quantal analysis of inhibitory synaptic transmission in the dentate gyrus of rat hippocampal slices: a patch-clamp study. J Physiol. 1990;430:213–249
  21. Connolly E, Worthley LI. Intravenous magnesium. Crit Care Resusc. 1999;1:162–172
  22. Gok B, Okutan O, Beskonakli E, Kilinc K. Effect of magnesium sulphate following spinal cord injury in rats. Chin J Physiol. 2007;50:93–97
  23. Narahashi T. Chemicals as tools in the study of excitable membrane. Physiol Res. 1974;54:813–889
  24. Yoshida S. Tetrodotoxin-resistant sodium channels. Cell Mol Neurobiol. 1994;14:227–244
  25. Rosenberg LJ, Wrathall JR. Time course studies on the effectiveness of tetrodotoxin in reducing consequences of spinal cord contusion. J Neurosci Res. 2001;66:191–202

PII: S0969-8051(08)00247-3

doi: 10.1016/j.nucmedbio.2008.11.011

Nuclear Medicine and Biology
Volume 36, Issue 2 , Pages 183-189 , February 2009