Nuclear Medicine and Biology
Volume 35, Issue 7 , Pages 755-761 , October 2008

In vitro evaluation, biodistribution and scintigraphic imaging in mice of radiolabeled anthrax toxins

  • Ekaterina Dadachova

      Affiliations

    • Department of Nuclear Medicine, Albert Einstein College of Medicine, Bronx, NY 10461, USA
    • Department of Microbiology and Immunology, Albert Einstein College of Medicine, Bronx, NY 10461, USA
    • Corresponding Author InformationCorresponding author. Department of Nuclear Medicine, Albert Einstein College of Medicine, Bronx, NY 10461, USA. Tel.: +1 718 405 8485; fax: +1 718 405 8457.
    • ,
  • Johanna Rivera

      Affiliations

    • Department of Microbiology and Immunology, Albert Einstein College of Medicine, Bronx, NY 10461, USA
    • ,
  • Ekaterina Revskaya

      Affiliations

    • Department of Nuclear Medicine, Albert Einstein College of Medicine, Bronx, NY 10461, USA
    • ,
  • Antonio Nakouzi

      Affiliations

    • Department of Microbiology and Immunology, Albert Einstein College of Medicine, Bronx, NY 10461, USA
    • ,
  • Sean M. Cahill

      Affiliations

    • Department of Biochemistry, Albert Einstein College of Medicine, Bronx, NY 10461, USA
    • ,
  • Michael Blumenstein

      Affiliations

    • Department of Chemistry and Biochemistry, Hunter College and the Graduate School of the City University of New York, NY 10021, USA
    • ,
  • Hui Xiao

      Affiliations

    • Laboratory for Macromolecular Analysis and Proteomics, Albert Einstein College of Medicine, Bronx, NY 10461, USA
    • ,
  • Dmitry Rykunov

      Affiliations

    • Department of Biochemistry, Albert Einstein College of Medicine, Bronx, NY 10461, USA
    • ,
  • Arturo Casadevall

      Affiliations

    • Department of Microbiology and Immunology, Albert Einstein College of Medicine, Bronx, NY 10461, USA
    • Department of Medicine, Albert Einstein College of Medicine, Bronx, NY 10461, USA

Received 19 May 2008 ,Revised 5 July 2008 ,Accepted 10 July 2008.

References 

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  2. Friedlander AM, Bhatnagar R, Leppla SH, Johnson L, Singh Y. Characterization of macrophage sensitivity and resistance to anthrax lethal toxin. Infect Immun. 1993;61:245–252
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  6. Rivera J, Nakouzi A, Abboud N, Revskaya E, Goldman D, Collier RJ, et al. A monoclonal antibody to Bacillus anthracis protective antigen defines a neutralizing epitope in domain 1. Infect Immun. 2006;74:4149–4156
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  8. Mukherjee S, Lee SC, Casadevall A. Antibodies to Cryptococcus neoformans glucuronoxylomannan enhance antifungal activity of murine macrophages. Infect Immun. 1995;63:573–579
  9. Leppla SH. Anthrax toxin edema factor: a bacterial adenylate cyclase that increases cyclic AMP concentrations of eukaryotic cells. Proc Natl Acad Sci U S A. 1982;79:3162–3166
  10. Lindmo T, Boven E, Cuttitta F, Fedorko J, Bunn PA. Determination of the immunoreactive fraction of radiolabeled monoclonal antibodies by linear extrapolation to binding at infinite antigen excess. J Immunol Methods. 1984;72:77–89
  11. Dadachova E, Bryan RA, Apostolidis C, Morgenstern A, Zhang T, Moadel T, et al. Interaction of radiolabeled antibodies with fungal cells and components of immune system in vitro and during radioimmunotherapy of experimental fungal infection. J Infect Dis. 2006;193:1427–1436
  12. Schweitzer AD, Rakesh V, Revskaya E, Datta A, Casadevall A, Dadachova E. Computational model predicts effective delivery of 188-Re-labeled melanin-binding antibody to the metastatic melanoma tumors with wide range of melanin concentrations. Melanoma Res. 2007;17:291–303
  13. Escuyer V, Collier RJ. Anthrax protective antigen interacts with a specific receptor on the surface of CHO-K1 cells. Infect Immun. 1991;59:3381–3386
  14. Moayeri M, Wiggins JF, Leppla SH. Anthrax protective antigen cleavage and clearance from the blood of mice and rats. Infect Immun. 2007;75:5175–5184
  15. Zuckier LS, Dohan O, Li Y, Carrasco N, Dadachova E. Kinetics of perrhenate uptake and comparative biodistribution of perrhenate, pertechnetate and iodide by NIS-expressing tissues in vivo. J Nucl Med. 2004;45:500–507
  16. Hay RV, Duesbery NS, Skinner RS, VanBrocklin MW, Vande Woude GF, Gross MD. Biodistribution of radiolabeled anthrax lethal factor in mice. In: Society of Nuclear Medicine 2005 Annual Meeting, Abst. # 1289. 2005;

PII: S0969-8051(08)00170-4

doi: 10.1016/j.nucmedbio.2008.07.001

Nuclear Medicine and Biology
Volume 35, Issue 7 , Pages 755-761 , October 2008

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