Nuclear Medicine and Biology
Volume 35, Issue 7 , Pages 733-740, October 2008

Sensitive determination of specific radioactivity of positron emission tomography radiopharmaceuticals by radio high-performance liquid chromatography with fluorescence detection

  • Ryuji Nakao

      Affiliations

    • Molecular Imaging Center, National Institute of Radiological Sciences, Chiba 263-8555, Japan
    • Corresponding Author InformationCorresponding author. Tel.: +81 43 206 4041; fax: +81 43 206 3261.
    • ,
  • Kenji Furutsuka

      Affiliations

    • Molecular Imaging Center, National Institute of Radiological Sciences, Chiba 263-8555, Japan
    • Sumitomo Accelerator Service, Tokyo 141-8686, Japan
    • ,
  • Masatoshi Yamaguchi

      Affiliations

    • Faculty of Pharmaceutical Sciences, Fukuoka University, Fukuoka 814-0180, Japan
    • ,
  • Kazutoshi Suzuki

      Affiliations

    • Molecular Imaging Center, National Institute of Radiological Sciences, Chiba 263-8555, Japan

Received 23 May 2008; received in revised form 12 June 2008; accepted 19 June 2008.

Abstract 

A sensitive quality control method is often required in positron emission tomography (PET) radiopharmaceutical analysis due to the high specific radioactivity of synthetic products. The applicability of a radio high-performance liquid chromatography (HPLC) method with fluorescence detection was evaluated for a wide variety of PET radiopharmaceuticals. In 29 different radiopharmaceuticals studied, 20 compounds exhibited native fluorescence. These properties enabled sensitive determination of their chemical masses by direct fluorimetric detection after separation by HPLC. For some substances, detection limits were below nanograms per milliliter level, at least 40 times better than current UV absorbance detection. Sufficient reproducibility and linearity were obtained for the analysis of pharmaceutical fluid. Post-column fluorimetric derivatization was also established for the quantitative determination of FDG and ClDG in [18F]FDG samples. These methods could be applied successfully to the analysis of PET radiopharmaceuticals with ultra-high specific radioactivity.

Keywords: PET radiopharmaceuticals, HPLC, Fluorescence detection, Specific radioactivity, Quality control

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PII: S0969-8051(08)00144-3

doi:10.1016/j.nucmedbio.2008.06.005

Nuclear Medicine and Biology
Volume 35, Issue 7 , Pages 733-740, October 2008

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