Comparison of intravenous and intraperitoneal [¹²³I]IBZM injection for dopamine D2 receptor imaging in mice

Abstract 

Introduction

Intraperitoneal (IP) injection represents an attractive alternative route of radiotracer administration for small animal imaging, e.g., for longitudinal studies in transgenic mouse models. We explored the cerebral kinetics of the reversible dopamine D2 receptor ligand [¹²³I]IBZM after IP injection in mice.

Methods

Cerebral [¹²³I]IBZM kinetics were assessed by ex vivo autoradiography in mice sacrificed between 30 and 200 min after IP or intravenous (IV) injection. The striatum-to-cerebellum (S/C) uptake ratio at 140 min was evaluated in wild-type mice and R6/2 transgenic mice (a Huntington’s disease model) in comparison with in vitro autoradiography using [³H]raclopride.

Results

[¹²³I]IBZM uptake was slower and lower after IP injection [maximum uptake in striatum 5.6% injected dose per gram (ID/g) at 60 min] than IV injection (10.5%ID/g at 30 min). Between 60 and 120 min, striatal (cerebellar) uptake after IP injection reached 63% (91%) of the uptake after IV injection. The S/C uptake ratio increased to 15.5 at 200 min after IP injection, which corresponds to 87% of the IV injection value (17.8). Consistent with in vitro [³H]raclopride autoradiography, the S/C ratio given by ex vivo [¹²³I]IBZM autoradiography (140 min after IP injection) was significantly reduced in R6/2 mice.

Conclusions

Although IP injection resulted in slower kinetics, relevant measures of dopamine D2 receptor availability were comparable. Thus, IP injection represents a promising route of tracer administration for small animal [¹²³I]IBZM SPECT. This should considerably simplify the implementation of longitudinal small animal neuroimaging studies, e.g., in transgenic mouse models.

Keywords: Small animal imaging, Intraperitoneal injection, [¹²³I]IBZM, Dopamine D2 receptor, SPECT