Nuclear Medicine and Biology
Volume 34, Issue 5 , Pages 471-478 , July 2007

Evaluation of 5′-deoxy-5′-[F-18]fluorothymidine as a tracer of intracellular thymidine phosphorylase activity

  • John R. Grierson

      Affiliations

    • Department of Radiology, University of Washington, Seattle, WA 98195, USA
    • Corresponding Author InformationCorresponding author. Fax: +1 206 543 8356.
    • ,
  • J. Scott Brockenbrough

      Affiliations

    • Department of Radiology, University of Washington, Seattle, WA 98195, USA
    • ,
  • Janet S. Rasey

      Affiliations

    • Department of Radiation Oncology, University of Washington, Seattle, WA 98195, USA
    • ,
  • Linda W. Wiens

      Affiliations

    • Department of Radiology, University of Washington, Seattle, WA 98195, USA
    • ,
  • Jeffery L. Schwartz

      Affiliations

    • Department of Radiation Oncology, University of Washington, Seattle, WA 98195, USA
    • ,
  • Robert Jordan

      Affiliations

    • Department of Radiation Oncology, University of Washington, Seattle, WA 98195, USA
    • ,
  • Hubert Vesselle

      Affiliations

    • Department of Radiology, University of Washington, Seattle, WA 98195, USA

Received 21 February 2007 ,Revised 7 March 2007 ,Accepted 13 March 2007.

References 

  1. Tozzi MG, Camici M, Mascia L, Sgarrella F, Ipata PL. Pentose phosphates in nucleoside interconversion and catabolism. FEBS J. 2006;273(6):1089–1101
  2. Toi M, Atiqur Rahman M, Bando H, Chow LW. Thymidine phosphorylase (platelet-derived endothelial-cell growth factor) in cancer biology and treatment. Lancet Oncol. 2005;6(3):158–166
  3. Langen P, Kowollik G. 5′-Deoxy-5′-fluorothymidine, a biochemical analogue of thymidine-5′-monophosphate selectively inhibiting DNA synthesis. Eur J Biochem. 1968;6:344–351
  4. Yau EK, Coward JK. Filter chromatography. Aldrichimica Acta. 1988;21(4):106–107
  5. Zhu HG, Lenzi M, Schwartz EL. The Sp1 transcription factor contributes to the tumor necrosis factor-induced expression of the angiogenic factor thymidine phosphorylase in human colon carcinoma cells. Oncogene. 2002;21:8477–8485
  6. Rasey JS, Grierson JR, Wiens LW, Kolb PD, Schwartz JL. Validation of FLT uptake as a measure of thymidine kinase-1 activity in A549 cells. J Nucl Med. 2002;43:1210–1217
  7. Vos MG, Visser GM, Pike VW, Davenport RJ, Vaalburg W. Synthesis of 5′-deoxy-5′-[18F]fluoro-thymidine (5′-[18F]FDT): a bifunctional agent to investigate DNA synthesis rate and a useful tool as a terminal building unit in antisense ODN labelling for PET. J Labelled Compd Radiopharm. 1995;37:338–340
  8. Panova NG, Alexeev C.S, Kuzmicho AS, Shcheveleva EV, Gavryushov SA, Polyakov KM, Substrate specificity of Escherichia coli thymidine phosphorylase. Biochemistry (Moscow), manuscript BM06-089; in press http://www.protein.bio.msu.ru/biokhimiya/inpress/pdf/BM06-089.pdf
  9. de Bruin M, Laan AC, Noordhuis P, Fukushima M, Hoekman K, Pinedo HM, et al. Rapid disappearance of deoxyribose-1-phosphate in platelet derived endothelial cell growth factor/thymidine phosphorylase overexpressing cells. Biochem Biophys Res Commun. 2003;301:675–679
  10. Bzowska A, Kulikowska E, Shugar D. Purine nucleoside phosphorylase: properties, functions and clinical aspects. Pharm Ther. 2000;88:349–425

PII: S0969-8051(07)00085-6

doi: 10.1016/j.nucmedbio.2007.03.004

Nuclear Medicine and Biology
Volume 34, Issue 5 , Pages 471-478 , July 2007

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