Nuclear Medicine and Biology
Volume 33, Issue 4 , Pages 529-534 , May 2006

Asialoglycoprotein-receptor-targeted hepatocyte imaging using 99mTc galactosylated chitosan

  • Eun-Mi Kim

      Affiliations

    • Department of Nuclear Medicine, Research Institute of Clinical Medicine, Chonbuk National University School of Medicine, Jeonju, Jeonbuk, Republic of Korea
    • ,
  • Hwan-Jeong Jeong

      Affiliations

    • Department of Nuclear Medicine, Research Institute of Clinical Medicine, Chonbuk National University School of Medicine, Jeonju, Jeonbuk, Republic of Korea
    • Corresponding Author InformationCorresponding author. Department of Nuclear Medicine, Chonbuk National University Hospital, Jeonju, Jellabuk-do 561-756, Republic of Korea. Tel.: +82 63 250 1674; fax: +82 63 250 1676.
    • ,
  • Se-Lim Kim

      Affiliations

    • Department of Nuclear Medicine, Research Institute of Clinical Medicine, Chonbuk National University School of Medicine, Jeonju, Jeonbuk, Republic of Korea
    • ,
  • Myung-Hee Sohn

      Affiliations

    • Department of Nuclear Medicine, Research Institute of Clinical Medicine, Chonbuk National University School of Medicine, Jeonju, Jeonbuk, Republic of Korea
    • ,
  • Jae-Woon Nah

      Affiliations

    • Division of Applied Materials Engineering, Department of Polymer Science and Engineering, Sunchon National University, Sunchon, Jeonnam, Republic of Korea
    • ,
  • Hee-Seung Bom

      Affiliations

    • Department of Nuclear Medicine, Chonnam National University School of Medicine, Gwangju, Republic of Korea
    • ,
  • In-Kyu Park

      Affiliations

    • School of Agricultural Biotechnology, Seoul National University, Seoul, Republic of Korea
    • ,
  • Chong-Su Cho

      Affiliations

    • School of Agricultural Biotechnology, Seoul National University, Seoul, Republic of Korea

Received 23 March 2005 ,Revised 13 March 2006 ,Accepted 13 March 2006.

References 

  1. Pricer WE, Ashwell G. The binding of desialylated glycoproteins by plasma membranes of liver. J Biol Chem. 1971;246:4825–4833
  2. Morell AG, Irvine RA, Sternlieb I, Scheinberg IH, Ashwell G. Physical and chemical studies on ceruloplasmin: V. Metabolic studies on sialic acid-free ceruloplasmin in vivo. J Biol Chem. 1968;243:155–159
  3. Spiess M. The asialoglycoprotein receptor: a model for endocytic transport receptors. Biochemistry. 1990;29:10009–10018
  4. Bettinger T, Remy JS, Erbacher P. Size reduction of galactosylated PEI/DNA complexes improves lectin-mediated gene transfer into hepatocytes. Bioconjug Chem. 1999;10:558–561
  5. Niidome T, Urakawa M, Sato H, Takahara Y, Anai T, Hatakayama T, et al. Gene transfer into hepatoma cells mediated by galactose-modified alpha-helical peptides. Biomaterials. 1999;21:1811–1819
  6. Sasaki N, Shiomi S, Iwata Y, Nishiguchi S, Kuroki T, Kawabe J, et al. Clinical usefulness of scintigraphy with 99mTc-galactosyl-human serum albumin for prognosis of cirrhosis of the liver. J Nucl Med. 1999;40:1652–1656
  7. Stadalnik RC, Vera DR, Woodle ES, Trudeau WL, Porter BA, Ward RE, et al. Technetium-99m NGA functional hepatic imaging: preliminary clinical experience. J Nucl Med. 1985;26:1233–1242
  8. Jeong JM, Hong MK, Lee J, Son M, So Y, Lee DS, et al. 99mTc-neolactosylated human serum albumin for imaging the hepatic asialoglycoprotein receptor. Bioconjug Chem. 2004;15:850–855
  9. Park IK, Kim TH, Park YH, Shin BA, Choi ES, Chowdhury EH, et al. Galactosylated chitosan-graft-poly(ethylene glycol) as hepatocyte-targeting DNA carrier. J Control Release. 2001;76:349–362
  10. Park IK, Yang J, Jeong HJ, Bom HS, Harada I, Akaike T, et al. Galactosylated chitosan as a synthetic extracellular matrix for hepatocytes attachment. Biomaterials. 2003;24:2331–2337
  11. Kim EM, Jeong HJ, Park IK, Cho CS, Kim CG, Bom HS. Hepatocyte-targeted nuclear imaging using 99mTc-galactosylated chitosan: conjugation, targeting, biodistribution. J Nucl Med. 2005;46:141–145
  12. Janet H. Methods of blood collection in the mouse. Lab Animal. 2000;29:47–53
  13. Saha GB. Fundamentals of nuclear pharmacy. 4th ed.. New York (USA): Springer-Verlag; 1998;
  14. Vera DR, Wallace AM, Hoh CK, Mattrey RF. A synthetic macromolecule for sentinel node detection: (99m)Tc–DTPA–mannosyl–dextran. J Nucl Med. 2001;42:951–959
  15. Mendez J, Wallace AM, Hoh CK, Vera DR. Detection of gastric and colonic sentinel nodes through endoscopic administration of 99mTc–DTPA–mannosyl–dextran in pigs. J Nucl Med. 2003;44:1677–1681
  16. Matsunaga K, Hara K, Imamura T, Fujioka T, Takata J, Karube Y. Technetium labeling of dextran incorporating cysteamine as a ligand. Nucl Med Biol. 2005;32(3):279–285
  17. Onishi H, Machida Y. Biodegradation and distribution of water-soluble chitosan in mice. Biomaterials. 1999;20:175–182

PII: S0969-8051(06)00061-8

doi: 10.1016/j.nucmedbio.2006.03.005

Nuclear Medicine and Biology
Volume 33, Issue 4 , Pages 529-534 , May 2006

Article Tools

* Image Usage & Resolution