Reproducibility of intraperitoneal 2-deoxy-2-[18F]-fluoro-d-glucose cerebral uptake in rodents through time

Marsteller, Douglas A.; Barbarich-Marsteller, Nicole C.; Fowler, Joanna S.; Schiffer, Wynne K.; Alexoff, David L.; Rubins, Daniel J.; Dewey, Stephen L.

doi:10.1016/j.nucmedbio.2005.09.003

« Previous Next »Nuclear Medicine and Biology
Volume 33, Issue 1 , Pages 71-79, January 2006

Reproducibility of intraperitoneal 2-deoxy-2-[¹⁸F]-fluoro-d-glucose cerebral uptake in rodents through time

Douglas A. Marsteller
- Graduate Program in Molecular and Cellular Pharmacology, SUNY Stony Brook, Stony Brook, NY 11794-8651, USA
- Chemistry Department, Brookhaven National Laboratory, Upton, NY 11973-5000, USA
- Corresponding author. Chemistry Department, Brookhaven National Laboratory, Upton, NY 11973-5000, USA. Tel.: +1 631 344 4393; fax: +1 631 344 5815.
Nicole C. Barbarich-Marsteller
- Chemistry Department, Brookhaven National Laboratory, Upton, NY 11973-5000, USA
- Graduate Program in Neuroscience, Department of Neurobiology and Behavior, SUNY Stony Brook, Stony Brook, NY 11794-5230, USA
Joanna S. Fowler
- Chemistry Department, Brookhaven National Laboratory, Upton, NY 11973-5000, USA
Wynne K. Schiffer
- Chemistry Department, Brookhaven National Laboratory, Upton, NY 11973-5000, USA
David L. Alexoff
- Chemistry Department, Brookhaven National Laboratory, Upton, NY 11973-5000, USA
Daniel J. Rubins
- Imaging Department, Merck Research Laboratories, West Point, PA 19486, USA
Stephen L. Dewey
- Graduate Program in Molecular and Cellular Pharmacology, SUNY Stony Brook, Stony Brook, NY 11794-8651, USA
- Chemistry Department, Brookhaven National Laboratory, Upton, NY 11973-5000, USA
- Graduate Program in Neuroscience, Department of Neurobiology and Behavior, SUNY Stony Brook, Stony Brook, NY 11794-5230, USA
- Psychiatry Department, New York University School of Medicine, New York, NY 10016, USA

Received 4 August 2005; received in revised form 7 September 2005; accepted 12 September 2005.

Abstract

Introduction

One strength of small animal imaging is the ability to obtain longitudinal measurements within the same animal, effectively reducing the number of animals needed and increasing statistical power. However, the variability of within-rodent brain glucose uptake after an intraperitoneal injection across an extended time has not been measured.

Methods

Small animal imaging with 2-deoxy-2-[¹⁸F]-fluoro-d-glucose (¹⁸FDG) was used to determine the variability of a 50-min brain ¹⁸FDG uptake following an intraperitoneal injection over time in awake male and female Sprague–Dawley rodents.

Results

After determining the variability of an intraperitoneal injection in the awake rat, we found that normalization of brain ¹⁸FDG uptake for (1) injected dose and body weight or (2) body weight, plasma glucose concentration and injected dose resulted in a coefficient of variation (CV) of 15%. However, if we normalized regional uptake to whole brain to compare relative regional changes, the CV was less than 5%. Normalized cerebral ¹⁸FDG uptake values were reproducible for a 2-week period in young adult animals. After 1 year, both male and female animals had reduced whole-brain uptake, as well as reduced regional hippocampal and striatal ¹⁸FDG uptake.

Conclusion

Overall, our results were similar to findings in previous rodent and human clinical populations; thus, using a high throughput study with intraperitoneal ¹⁸FDG is a promising preclinical model for clinical populations. This is particularly relevant for measuring changes in brain function after experimental manipulation, such as long-term pharmacological administration.

Keywords: Age, Glucose utilization, Positron emission tomography, Small animal imaging, Sprague–Dawley, ¹⁸FDG