Effects of anpirtoline on regional serotonin synthesis in the rat brain: an autoradiographic study

Abstract 

Anpirtoline has been described as an agonist at 5-HT_1B receptors with a relatively high potency. It also acts as an agonist at 5-HT_1A receptors, but has a lower potency than at the 5-HT_1B sites. There is very little known about the mechanism by which anpirtoline influences regional 5-HT synthesis. The aim of the present study was to investigate the effects of acutely and chronically administered anpirtoline on 5-HT synthesis in the rat brain using the autoradiographic α-[¹⁴C]methyl-l-tryptophan method. In the acute study, anpirtoline (2.0 mg/kg) was administered intraperitoneally 30 min before the tracer injection. The control rats were injected with the same volume of saline. In the chronic study, anpirtoline (2 mg/kg per day) was injected subcutaneously in saline once a day for 10 days. There were no significant differences between the plasma-free and total tryptophan concentrations between the anpirtoline treatment and the respective control groups. In the acute experiment, 5-HT synthesis rates in all of the brain areas investigated were significantly decreased by anpirtoline when compared to the saline-treated group. In the chronic anpirtoline experiment, 5-HT synthesis rates of almost all of the projection areas, as well as the raphe nuclei, were normalized or had a tendency to be normalized. These results suggest that it is likely that the terminal 5-HT_1B receptors are involved in the regulation of 5-HT synthesis in the projection areas and that 5-HT synthesis, in the raphe, is likely influenced by anpirtoline's 5-HT_1A and/or 5-HT_1B agonistic properties.

Keywords: Alpha-methyl-l-tryptophan, Autoradiography, Serotonin synthesis rate, Anpirtoline, 5-HT_1B agonist